Insights into SARS-CoV transcription and replication from the structure of the nsp7–nsp8 hexadecamer
Identifieur interne : 004B73 ( Main/Exploration ); précédent : 004B72; suivant : 004B74Insights into SARS-CoV transcription and replication from the structure of the nsp7–nsp8 hexadecamer
Auteurs : Yujia Zhai [République populaire de Chine] ; Fei Sun [République populaire de Chine] ; Xuemei Li [République populaire de Chine] ; Hai Pang [République populaire de Chine] ; Xiaoling Xu [République populaire de Chine] ; Mark Bartlam [République populaire de Chine] ; Zihe Rao [République populaire de Chine]Source :
- Nature Structural & Molecular Biology [ 1545-9993 ] ; 2005-11.
Descripteurs français
- KwdFr :
- Amorces ADN, Analyse de séquence d'ADN, Complexes multiprotéiques (), Complexes multiprotéiques (métabolisme), Complexes multiprotéiques (ultrastructure), Cristallisation, Données de séquences moléculaires, Microscopie électronique, Modèles moléculaires, Protéines virales non structurales (), Protéines virales non structurales (métabolisme), Protéines virales non structurales (ultrastructure), RNA replicase (métabolisme), Relation structure-activité, Réplication virale (génétique), Réplication virale (physiologie), Séquence d'acides aminés, Séquence nucléotidique, Test de retard de migration électrophorétique, Transcription génétique (génétique), Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- génétique : Réplication virale, Transcription génétique, Virus du SRAS.
- métabolisme : Complexes multiprotéiques, Protéines virales non structurales, RNA replicase.
- physiologie : Réplication virale, Virus du SRAS.
- ultrastructure : Complexes multiprotéiques, Protéines virales non structurales.
- Amorces ADN, Analyse de séquence d'ADN, Complexes multiprotéiques, Cristallisation, Données de séquences moléculaires, Microscopie électronique, Modèles moléculaires, Protéines virales non structurales, Relation structure-activité, Séquence d'acides aminés, Séquence nucléotidique, Test de retard de migration électrophorétique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Crystallization, DNA Primers, Electrophoretic Mobility Shift Assay, Microscopy, Electron, Models, Molecular, Molecular Sequence Data, Multiprotein Complexes (chemistry), Multiprotein Complexes (metabolism), Multiprotein Complexes (ultrastructure), RNA Replicase (metabolism), SARS Virus (genetics), SARS Virus (physiology), Sequence Analysis, DNA, Structure-Activity Relationship, Transcription, Genetic (genetics), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (metabolism), Viral Nonstructural Proteins (ultrastructure), Virus Replication (genetics), Virus Replication (physiology).
- MESH :
- chemical , chemistry : Multiprotein Complexes, Viral Nonstructural Proteins.
- chemical , metabolism : Multiprotein Complexes, RNA Replicase, Viral Nonstructural Proteins.
- chemical , ultrastructure : Multiprotein Complexes, Viral Nonstructural Proteins.
- chemical : DNA Primers.
- genetics : SARS Virus, Transcription, Genetic, Virus Replication.
- physiology : SARS Virus, Virus Replication.
- Amino Acid Sequence, Base Sequence, Crystallization, Electrophoretic Mobility Shift Assay, Microscopy, Electron, Models, Molecular, Molecular Sequence Data, Sequence Analysis, DNA, Structure-Activity Relationship.
Abstract
Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7–nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-Å resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of ∼30 Å, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase.
Url:
- https://api.istex.fr/ark:/67375/GT4-Z0QMZZ58-N/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096913
DOI: 10.1038/nsmb999
Affiliations:
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de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nature Structural & Molecular Biology</title><idno type="ISSN">1545-9993</idno><idno type="eISSN">1545-9985</idno><imprint><publisher>Nature Publishing Group</publisher><date when="2005-11">2005-11</date><biblScope unit="vol">12</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="980">980</biblScope><biblScope unit="page" to="986">986</biblScope><date type="Copyright" when="2005">2005</date></imprint><idno type="ISSN">1545-9993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1545-9993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Crystallization</term><term>DNA Primers</term><term>Electrophoretic Mobility Shift Assay</term><term>Microscopy, Electron</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Multiprotein Complexes (chemistry)</term><term>Multiprotein Complexes (metabolism)</term><term>Multiprotein Complexes (ultrastructure)</term><term>RNA Replicase (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Sequence Analysis, DNA</term><term>Structure-Activity Relationship</term><term>Transcription, Genetic (genetics)</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Viral Nonstructural Proteins (ultrastructure)</term><term>Virus Replication (genetics)</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN</term><term>Analyse de séquence d'ADN</term><term>Complexes multiprotéiques ()</term><term>Complexes multiprotéiques (métabolisme)</term><term>Complexes multiprotéiques (ultrastructure)</term><term>Cristallisation</term><term>Données de séquences moléculaires</term><term>Microscopie électronique</term><term>Modèles moléculaires</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (métabolisme)</term><term>Protéines virales non structurales (ultrastructure)</term><term>RNA replicase (métabolisme)</term><term>Relation structure-activité</term><term>Réplication virale (génétique)</term><term>Réplication virale (physiologie)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test de retard de migration électrophorétique</term><term>Transcription génétique (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Multiprotein Complexes</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Multiprotein Complexes</term><term>RNA Replicase</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Multiprotein Complexes</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Transcription, Genetic</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Réplication virale</term><term>Transcription génétique</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Complexes multiprotéiques</term><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réplication virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Complexes multiprotéiques</term><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Crystallization</term><term>Electrophoretic Mobility Shift Assay</term><term>Microscopy, Electron</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Analyse de séquence d'ADN</term><term>Complexes multiprotéiques</term><term>Cristallisation</term><term>Données de séquences moléculaires</term><term>Microscopie électronique</term><term>Modèles moléculaires</term><term>Protéines virales non structurales</term><term>Relation structure-activité</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test de retard de migration électrophorétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7–nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-Å resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of ∼30 Å, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhai, Yujia" sort="Zhai, Yujia" uniqKey="Zhai Y" first="Yujia" last="Zhai">Yujia Zhai</name></noRegion><name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name><name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name><name sortKey="Li, Xuemei" sort="Li, Xuemei" uniqKey="Li X" first="Xuemei" last="Li">Xuemei Li</name><name sortKey="Li, Xuemei" sort="Li, Xuemei" uniqKey="Li X" first="Xuemei" last="Li">Xuemei Li</name><name sortKey="Pang, Hai" sort="Pang, Hai" uniqKey="Pang H" first="Hai" last="Pang">Hai Pang</name><name sortKey="Pang, Hai" sort="Pang, Hai" uniqKey="Pang H" first="Hai" last="Pang">Hai Pang</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Sun, Fei" sort="Sun, Fei" uniqKey="Sun F" first="Fei" last="Sun">Fei Sun</name><name sortKey="Sun, Fei" sort="Sun, Fei" uniqKey="Sun F" first="Fei" last="Sun">Fei Sun</name><name sortKey="Xu, Xiaoling" sort="Xu, Xiaoling" uniqKey="Xu X" first="Xiaoling" last="Xu">Xiaoling Xu</name><name sortKey="Xu, Xiaoling" sort="Xu, Xiaoling" uniqKey="Xu X" first="Xiaoling" last="Xu">Xiaoling Xu</name><name sortKey="Zhai, Yujia" sort="Zhai, Yujia" uniqKey="Zhai Y" first="Yujia" last="Zhai">Yujia Zhai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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